IMMUNOHISTOCHEMICAL EXPRESSION OF CARDIAC MUSCLE TISSUE IN RELATION TO AGE: AN EXPERIMENTAL STUDY
Abstract
The aim of this study was to investigate age-related features of the immunohistochemical expression of structural proteins in the cardiac muscle of white outbred rats. The experiment was conducted on two age groups: 2-month-old and 4-month-old animals. The left ventricular myocardium was analyzed using histological and immunohistochemical methods with antibodies against troponin I, α-actin, and desmin. A quantitative assessment of immunopositive cardiomyocytes and staining intensity was performed.
The results showed a significant age-dependent increase in the expression of troponin I, α-actin, and desmin, accompanied by enlargement of cardiomyocytes, dense organization of myofibrils, and enhanced myocardial vascularization. It was found that 4-month-old rats exhibit a more mature morphofunctional organization of the cardiac muscle compared to 2-month-old animals. These findings indicate a regular age-related differentiation of cardiomyocytes and can be used for evaluating postnatal myocardial development and modeling pathological conditions of the heart.
Keywords
myocardium, cardiomyocytes, immunohistochemistry, troponin I, α-actin, desmin, white outbred rats, age-related features
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