IN VITRO PROPAGATION FROM SEEDS OF NIGELLA SATIVA L. AND RUBIA TINCTORUM L.

Abstract

 The article presents the results in vitro propagation of two types of the medicinal plants, such as Black seeds belonging to the genus Nigella sativa L., family Ranunculaceae and Dyer's madder belonging to the genus Rubia tinctorum  L., family Rubiaceae in laboratory conditions. These cultures are used to grow from seeds in a nutrient medium. A nutrient medium with the following composition was prepared for the in vitro cultivation of Nigella sativa L. The composition of the nutrient medium was as follows: KNO₃-950 mg/l; NH₄NO₃-412 mg/l; MgSO₄∙7H₂O-185 mg/l; CaCl₂∙2H2O-440 mg/l; KH₂PO₄-68 mg/l; Fe-chelate, trace elements, mesoinositol - C₆H₁₂O₆ - 50 mg/l; thiamine - C₁₂H₁₇N₄OS∙HCl - 0.2 mg/l; nicotinic acid - C₆H₅NO₂ - 0.2 mg/l; pyridoxine - C₈H₁₁NO₃∙HCl - 0.2 mg/l; indolyl acetic acid - C₁₀H₉NO₂ - 0.5 mg/g; ferulic acid - 1 mg/l; It consists of sucrose - C₁₂H₂₂O₁₁ - 10 g/l, agar-agar - (C₁₂H₁₈O₉)n - 7.5 g/l, and the nutrient medium pH is 5,8.