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Vol. 13 No. 4 (2026)
International Multidisciplinary Journal for Research & Development

Articles

DETERMINATION OF HYDROPHILIC VITAMINS IN ALMOND FLOWERS OF THE “BOʻSTONLIQ” VARIETY USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

Published 2026-04-16

  • Asqarov Ibrohimjon Rahmonovich,Mo‘minjonov Mirjalol Muqimjon o‘g‘li,Jo‘raboyeva Gulhayo Akmaljon qizi

Asqarov Ibrohimjon Rahmonovich,Mo‘minjonov Mirjalol Muqimjon o‘g‘li,Jo‘raboyeva Gulhayo Akmaljon qizi
Professor, Doctor of Chemical Sciences, Department of Chemistry, Andijan State University, Honored Inventor of Uzbekistan, Chairman of the Academy of TABOBAT of Uzbekistan,Professor, Doctor of Chemical Sciences, Department of Chemistry, Andijan State University,Master’s Student, Chemistry Program, Andijan State University

Abstract

This study presents a detailed quantitative analysis of hydrophilic vitamins in almond (Prunus amygdalus) flowers of the “Boʻstonliq” variety using high-performance liquid chromatography (HPLC). Water-soluble vitamins, including B-group vitamins and vitamin C, were identified and quantified employing a Shim-pack GIST C18 column and a gradient mobile phase system.

The results revealed the presence of vitamins B1, B2, B3, B6, B9, PP (niacinamide), and C in the extract. Among them, vitamin B3 (10.165 mg/100 g), vitamin PP (6.063 mg/100 g), and vitamin B9 (5.258 mg/100 g) were found in relatively high concentrations, while vitamins B1, B2, B6, and C were detected in smaller amounts. Vitamin B12 was not detected in the analyzed samples.

The obtained results demonstrate that almond flowers are a rich source of biologically active compounds and can serve as a promising raw material for the development of functional foods and nutraceutical formulations.

Keywords

Almond flower, Prunus amygdalus, hydrophilic vitamins, B-complex vitamins, vitamin C, HPLC, gradient elution, bioactive compounds

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