ADVANCED HISTOLOGICAL TECHNIQUES FOR MICROSCOPIC EVALUATION OF HUMAN EPITHELIAL AND CONNECTIVE TISSUE STRUCTURES

Abstract

Histological examination is a fundamental method for studying the microscopic organization of human tissues and understanding their functional relationships. Recent advancements in tissue processing, staining techniques, and digital microscopy have significantly enhanced the accuracy of morphological assessment. The present study evaluates the effectiveness of advanced histological techniques in the microscopic analysis of epithelial and connective tissues. Standard paraffin-embedded tissue samples were stained using Hematoxylin and Eosin, Masson’s Trichrome, and Periodic Acid–Schiff methods. The findings demonstrate that specialized staining techniques provide improved differentiation of cellular and extracellular components compared to routine staining alone. The integration of digital imaging technologies further enhances structural visualization and analytical precision. These results confirm the importance of modern histological approaches in both biomedical research and diagnostic practice.