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Vol. 12 No. 05 (2025)
International Multidisciplinary Journal for Research & Development

Articles

IN VITRO MICROPROPAGATION OF AJUGA TURKESTANICA (REGEL) BRIQ.: A PATHWAY TO PATHOGEN-FREE SEEDLINGS

Published 2025-05-07

  • Mannabova M.M., Kushiev Kh.Kh., Bakeyev R.

Mannabova M.M., Kushiev Kh.Kh., Bakeyev R.
Research Institute Agrobiotechnologies and Biochemistry of Gulistan State University

Abstract

In recent years, the application of in vitro techniques has become a cornerstone in the conservation and propagation of plant biodiversity. This study focuses on the micropropagation of Ajuga Turkestanica, a medicinal plant species, under in vitro conditions. The research aimed to identify the optimal nutrient media and growth regulators for the efficient propagation of pathogen-free seedlings. The results demonstrated that the B5 nutrient medium supplemented with 0.1 µM BAP (6-Benzylaminopurine) was the most effective for the proliferation of Ajuga Turkestanica. Additionally, the BDS medium enriched with 5.0 µM BAP and 2.0 µM NAA (Naphthalene Acetic Acid) showed high efficiency in promoting shoot regeneration. The study also highlighted the importance of cytokinins, particularly BAP, over other growth regulators like TDZ (Thidiazuron) and Kinetin in the multiplication phase.

Keywords

Ajuga Turkestanica, in vitro, micropropagation, callus, MS medium, phytohormones.

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