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Vol. 12 No. 10 (2025)
International Multidisciplinary Journal for Research & Development

Articles

ANTIFUNGAL ACTIVITY OF ENDOPHYTIC BACTERIA FROM LYCIUM BARBARUM AND THEIR EFFECTS ON VEGETABLE CROPS

Published 2025-11-02

  • Abdumajitova Shakhlo

Abdumajitova Shakhlo
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Abstract

 Endophytic bacteria are beneficial microorganisms that inhabit the internal tissues of plants without causing harm, often producing bioactive compounds that suppress plant pathogens and enhance plant growth. The present study aimed to isolate, identify, and evaluate the antifungal activity of endophytic bacteria from Lycium barbarum and assess their effects on the growth of vegetable crops. A total of 18 bacterial isolates were obtained from the leaves, stems, and roots of L. barbarum. Molecular identification using 16S rRNA gene sequencing revealed that the isolates mainly belonged to the genera Bacillus, Pseudomonas, Streptomyces, and Enterobacter. Dual culture assays demonstrated that Bacillus subtilis (LB1) and Pseudomonas fluorescens (LB2) exhibited strong antifungal activity, inhibiting the growth of Fusarium oxysporum, Alternaria solani, and Botrytis cinerea by 60–65%. Inoculation of vegetable seeds (tomato, cucumber, and pepper) with selected isolates significantly improved germination rate, root and shoot length, and fresh biomass compared to control plants (p < 0.05). These findings indicate that endophytic bacteria from Lycium barbarum possess both antifungal and plant growth-promoting properties, suggesting their potential as eco-friendly biocontrol agents and biofertilizers for sustainable crop management.

Keywords

Endophytic bacteria; Lycium barbarum; antifungal activity; Bacillus subtilis; Pseudomonas fluorescens; vegetable crops; biological control; plant growth promotion.

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